Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.


Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.


In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.


Illumina HiSeq Sequencing in High-Output mode


Internal (Partners HealthCare) External Academic Industry
Single 50 $1280 $1843 $2304
Paired 50 $2130 $3067 $3834
Paired 75 $2400 $3456 $4320
Single 100 $1790 $2578
Paired 100 $2750 $3960 $4950
Paired 25 $1640 $2362 $2952



Illumina HiSeq sequencing in Rapid mode

Illumina HiSeq2500 can perfom runs in Rapid mode, which are much faster but produce somewhat fewer reads (100-120 million reads per lane, two lanes per run). If both lanes of a rapid run include the same pool of samples, then cluster generation can be perfomed directly by HiSeq (on-board cluster generation, OBCG option). Otherwise, prior to the run we perform a separate step of cluster generation in the two lanes using the cBot instrument, similar to High-Output mode (cBot Duo option).


Internal (Partners HealthCare) External Academic Industry
Single 50 cBot Duo $1300 $1872 $2340
Single 50 OBCG $1130 $1627 $2034
Paired 50 cBot Duo $1940 $2794 $3492
Paired 50 OBCG $1760 $2534 $3168
Single 100 cBot Duo $1600 $2304 $2880
Single 100 OBCG $1370 $1973 $2466
Paired 100 cBot Duo $2500 $3600 $4500
Paired 100 OBCG $2250 $3240 $4050
Single 150 cBot Duo $1870 $2693 $3366
Single 150 OBCG $1620 $2333 $2916
Paired 150 cBot Duo $3090 $4450 $5562
Paired 150 OBCG $2940 $4234 $5292
Paired 25 cBot Duo $2090 $3010 $3762
Paired 25 OBCG $1770 $2549 $3186



Illumina MiSeq Sequencing

MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.


Internal (Partners HealthCare) External Academic Industry
Instrument operation by technician $135 $194 $244
MiSeq usage (cost per hour) $27 $39 $50



Library Quality Control after the first library


Internal (Partners HealthCare) External Academic Industry
Price per library $57 $82 $104



Single-Cell RNA-seq Library Construction (10x Genomics Chromium)


Internal (Partners HealthCare) External Academic Industry
Price per sample $1654 $2382 $2979


Library Construction


Option Total cost per sample, MULTI PLEXED External Academic Industry Total cost per sample, DUAL INDEX External Academic Industry
NEBNext Ultra II DNA Library Prep Kit for Illumina $145 $209 $261 $125 $180 $225
NEBNext PolyA + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina


$320 $400 N/A N/A N/A
NEBNext rRNA Depletion Kit + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina


$429 $536 N/A N/A


NEBNext Multiplex Small RNA Library Prep Set for Illumina $243 $345 $437 N/A N/A N/A
TruSeq Small RNA Sample Prep Kit $290 $418 $522 N/A N/A N/A
NEBNext Single Cell/Low Input RNA Library Prep Kit For Illumina $253 $364 $455 N/A N/A N/A
Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis $356 $513 $641 N/A N/A N/A
Fragmentation by Covaris $79 $114 $142 N/A N/A N/A