PRICING
Overview
Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.
Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.
In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.
Illumina NextSeq 2000 sequencing
Internal (MGB) | External Academic | Industry | ||||
---|---|---|---|---|---|---|
Option | Cost per run | Cost per million reads | Cost per run | Cost per million reads | Cost per run | Cost per million reads |
P3 SR50 | $2792 | $2.54 | $4021 | $3.66 | $5026 | $4.58 |
P3 SR100 | $4026 | $3.66 | $5798 | $5.27 | $7247 | $6.59 |
P3 SR200 | $5718 | $5.20 | $8234 | $7.49 | $10293 | $9.36 |
P3 PE25 | $2818 | $2.56 | $4058 | $3.69 | $5073 | $4.61 |
P3 PE50 | $4051 | $3.68 | $5834 | $5.30 | $7292 | $6.63 |
P3 PE100 | $5744 | $5.22 | $8272 | $7.52 | $10340 | $9.40 |
P3 PE150 | $7694 | $6.99 | $11080 | $10.07 | $13850 | $12.59 |
P2 SR100 | $2078 | $4.72 | $2993 | $6.80 | $3741 | $8.50 |
P2 SR200 | $3712 | $8.44 | $5346 | $12.16 | $6682 | $15.20 |
P2 PE50 | $2104 | $4.78 | $3030 | $6.89 | $3783 | $8.61 |
P2 PE100 | $3738 | $8.5 | $5383 | $12.24 | $6729 | $15.3 |
P2 PE150 | $4980 | $11.32 | $7172 | $16.31 | $8964 | $20.38 |
Illumina MiSeq Sequencing
MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Instrument operation by technician | $158 | $278 | $285 |
MiSeq usage (cost per hour) | $21 | $31 | $38 |
Library Quality Control after the first library
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per library | $61 | $88 | $110 |
Single-Cell RNA-seq Library Construction (10x Genomics Chromium, not including chip surcharge)
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $1940 | $2794 | $3492 |
10x Single-Cell ATAC-Seq library construction (not including chip surcharge and nuclei extraction)
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $2187 | $3150 | $3937 |
10x CITE-Seq (not including chip surcharge)
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $2063 | $2971 | $3714 |
10x Single-Cell Multiome ATAC-seq + Gene Expression library construction (not including chip surcharge and nuclei extraction)
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $3764 | $5421 | $6776 |
10x chip surcharge
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $444 | $640 | $800 |
Extraction of nuclei using 10x Genomics workflow
Internal (Partners HealthCare) | External Academic | Industry | |
---|---|---|---|
Price per sample | $290 | $418 | $522 |
Library Construction
Option | Total cost per sample, MULTI PLEXED | External Academic | Industry |
---|---|---|---|
NEBNext Ultra II DNA Library Prep Kit for Illumina | $157 | $227 | $283 |
NEBNext PolyA + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina | $222 |
$320 | $400 |
NEBNext rRNA Depletion Kit + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina | $275 |
$396 | $495 |
NEBNext Multiplex Small RNA Library Prep Set for Illumina | $219 | $316 | $395 |
NEBNext Single Cell/Low Input RNA Library Prep Kit For Illumina | $243 | $350 | $438 |
Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis | $370 | $533 | $666 |
Fragmentation by Covaris | $81 | $117 | $146 |
Thruplex low-input DNA library | $174 | $251 | $314 |
Clontech Pico RNA library construction | $390 | $562 | $702 |