MGHwebsite

PRICING

Overview

 

Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.

 

Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.

 

In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.

Illumina NextSeq 2000 sequencing

 

Internal (MGB) External Academic Industry
Option Cost per run Cost per million reads Cost per run Cost per million reads Cost per run Cost per million reads
P3 SR50 $2792 $2.54 $4021 $3.66 $5026 $4.58
P3 SR100 $4026 $3.66 $5798 $5.27 $7247 $6.59
P3 SR200 $5718 $5.20 $8234 $7.49 $10293 $9.36
P3 PE25 $2818 $2.56 $4058 $3.69 $5073 $4.61
P3 PE50 $4051 $3.68 $5834 $5.30 $7292 $6.63
P3 PE100 $5744 $5.22 $8272 $7.52 $10340 $9.40
P3 PE150 $7694 $6.99 $11080 $10.07 $13850 $12.59
P2 SR100 $2078 $4.72 $2993 $6.80 $3741 $8.50
P2 SR200 $3712 $8.44 $5346 $12.16 $6682 $15.20
P2 PE50 $2104 $4.78 $3030 $6.89 $3783 $8.61
P2 PE100 $3738 $8.5 $5383 $12.24 $6729 $15.3
P2 PE150 $4980 $11.32 $7172 $16.31 $8964 $20.38

 

 

Illumina MiSeq Sequencing

MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.

 

Internal (Partners HealthCare) External Academic Industry
Instrument operation by technician $158 $278 $285
MiSeq usage (cost per hour) $21 $31 $38

 

 

Library Quality Control after the first library

 

Internal (Partners HealthCare) External Academic Industry
Price per library $61 $88 $110

 

 

Single-Cell RNA-seq Library Construction (10x Genomics Chromium, not including chip surcharge)

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $1940 $2794 $3492

 

 

10x Single-Cell ATAC-Seq library construction (not including chip surcharge and nuclei extraction)

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $2187 $3150 $3937

 

 

10x CITE-Seq (not including chip surcharge)

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $2063 $2971 $3714

 

 

10x Single-Cell Multiome ATAC-seq + Gene Expression library construction (not including chip surcharge and nuclei extraction)

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $3764 $5421 $6776

 

 

10x chip surcharge

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $444 $640 $800

 

 

Extraction of nuclei using 10x Genomics workflow

 

Internal (Partners HealthCare) External Academic Industry
Price per sample $290 $418 $522

 


Library Construction

 

Option Total cost per sample, MULTI PLEXED External Academic Industry
NEBNext Ultra II DNA Library Prep Kit for Illumina $157 $227 $283
NEBNext PolyA + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

$222

$320 $400
NEBNext rRNA Depletion Kit + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

$275

$396 $495
NEBNext Multiplex Small RNA Library Prep Set for Illumina $219 $316 $395
NEBNext Single Cell/Low Input RNA Library Prep Kit For Illumina $243 $350 $438
Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis $370 $533 $666
Fragmentation by Covaris $81 $117 $146
Thruplex low-input DNA library $174 $251 $314
Clontech Pico RNA library construction $390 $562 $702