MGHwebsite

PRICING

Overview

 

Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.

 

Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.

 

In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.

 

Illumina HiSeq Sequencing in High-Output mode

 

Internal (Partners HealthCare) External Academic Industry
Single 50 $1290 $1858 $2322
Paired 50 $2000 $2880 $3600
Paired 75 $2340 $3370 $4212
Single 100 $1660 $2390 $2988
Paired 100 $2610 $3758 $4698
Paired 25 $1570 $2261 $2826

 

 

Illumina HiSeq sequencing in Rapid mode

Illumina HiSeq2500 can perfom runs in Rapid mode, which are much faster but produce somewhat fewer reads (100-120 million reads per lane, two lanes per run). If both lanes of a rapid run include the same pool of samples, then cluster generation can be perfomed directly by HiSeq (on-board cluster generation, OBCG option). Otherwise, prior to the run we perform a separate step of cluster generation in the two lanes using the cBot instrument, similar to High-Output mode (cBot Duo option).

 

Internal (Partners HealthCare) External Academic Industry
Single 50 cBot Duo $1520 $2189 $2736
Single 50 OBCG $1280 $1843 $2304
Paired 50 cBot Duo $2130 $3067 $3834
Paired 50 OBCG $1920 $2765 $3456
Single 100 cBot Duo $2110 $3038 $3798
Single 100 OBCG $1820 $2621 $3276
Paired 100 cBot Duo $2830 $4075 $5094
Paired 100 OBCG $2610 $3758 $4698
Single 150 cBot Duo $2480 $3571 $4464
Single 150 OBCG $2170 $3125 $3906
Paired 150 cBot Duo $4220 $6077 $7596
Paired 150 OBCG $4000 $5760 $7200
Paired 25 cBot Duo $1920 $2765 $3456
Paired 25 OBCG $1620 $2333 $2916

 

 

Illumina MiSeq Sequencing

MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.

 

Internal (Partners HealthCare) External Academic Industry
Instrument operation by technician $122 $176 $220
MiSeq usage (cost per hour) $23 $33 $42

 

 

Library Quality Control after the first library

 

Internal (Partners HealthCare) External Academic Industry
Price per library $47 $68 $85

 

 

Library Construction

 

Option Total cost per sample, SINGLE External Academic Industry Total cost per sample, MULTI PLEXED External Academic Industry Total cost per sample, DUAL INDEX External Academic Industry
NEBNext Ultra DNA Library Prep Kit for Illumina $152 $219 $274 $152 $219 $274 $153 $220 $275
NEBNext DNA Library Prep Master Mix Set for Illumina $165 $238 $297 $165 $238 $296 $165 $238 $297
PolyA selection + NEBNext Ultra Directional RNA Library Prep Kit for Illumina $193 $278 $347 $193 $278 $347 $193 $278 $348
RiboZero + NEBNext Ultra Directional RNA Library Prep Kit for Illumina $290 $418 $522 $290 $418 $522 $290 $418 $523
PolyA selection + NEBNext Ultra RNA Library Prep Kit for Illumina $189 $272 $340 $189 $272 $340 $189 $272 $340
RiboZero + NEBNext Ultra RNA Library Prep Kit for Illumina $286 $412 $514 $286 $412 $514 $286 $412 $515
NEBNext Multiplex Small RNA Library Prep Set for Illumina $199 $287 $358 $199 $287 $358 N/A N/A N/A
TruSeq Small RNA Sample Prep Kit $295 $425 $532 N/A N/A N/A N/A N/A N/A
Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis $311 $448 $560 N/A N/A N/A N/A N/A N/A
Fragmentation by Fragmentase $44 $63 $79 N/A N/A N/A N/A N/A N/A
Fragmentation by Covaris $45 $65 $82 N/A N/A N/A N/A N/A N/A