MGHwebsite

PRICING

Overview

 

Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.

 

Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.

 

In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.

 

Illumina HiSeq Sequencing in High-Output mode

 

Internal (Partners HealthCare) External Academic Industry
Single 50 $1400 $2016 $2520
Paired 50 $1970 $2837 $3546
Paired 75 $2590 $3730 $4662
Single 100 $1640 $2362 $2952
Paired 100 $2570 $3701 $4626
Paired 25 $1550 $2232 $2790

 

 

Illumina HiSeq sequencing in Rapid mode

Illumina HiSeq2500 can perfom runs in Rapid mode, which are much faster but produce somewhat fewer reads (100-120 million reads per lane, two lanes per run). If both lanes of a rapid run include the same pool of samples, then cluster generation can be perfomed directly by HiSeq (on-board cluster generation, OBCG option). Otherwise, prior to the run we perform a separate step of cluster generation in the two lanes using the cBot instrument, similar to High-Output mode (cBot Duo option).

 

Internal (Partners HealthCare) External Academic Industry
Single 50 cBot Duo $1820 $2621 $3276
Single 50 OBCG $1490 $2146 $2682
Paired 50 cBot Duo $2560 $3686 $4608
Paired 50 OBCG $2270 $3269 $4086
Single 100 cBot Duo $2140 $3082 $3852
Single 100 OBCG $1850 $2664 $3330
Paired 100 cBot Duo $3040 $4378 $5472
Paired 100 OBCG $2760 $3974 $4968
Single 150 cBot Duo $2500 $3600 $4500
Single 150 OBCG $2190 $3154 $3942
Paired 150 cBot Duo $3740 $5386 $6732
Paired 150 OBCG $3510 $5054 $6318
Paired 25 cBot Duo $1960 $2822 $3528
Paired 25 OBCG $1650 $2376 $2970

 

 

Illumina MiSeq Sequencing

MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.

 

Internal (Partners HealthCare) External Academic Industry
Instrument operation by technician $119 $171 $213
MiSeq usage (cost per hour) $23 $33 $42

 

 

Library Quality Control after the first library

 

Internal (Partners HealthCare) External Academic Industry
Price per library $50 $72 $90

 

 

Library Construction

 

Option Total cost per sample, SINGLE External Academic Industry Total cost per sample, MULTI PLEXED External Academic Industry Total cost per sample, DUAL INDEX External Academic Industry
NEBNext Ultra DNA Library Prep Kit for Illumina $149 $215 $268 $149 $215 $268 $149 $215 $268
NEBNext DNA Library Prep Master Mix Set for Illumina $161 $232 $290 $161 $232 $290 $162 $233 $290
PolyA selection + NEBNext Ultra Directional RNA Library Prep Kit for Illumina $189 $272 $341 $189 $272 $341 $190 $274 $342
RiboZero + NEBNext Ultra Directional RNA Library Prep Kit for Illumina $283 $408 $509 $282 $406 $508 $283 $408 $509
PolyA selection + NEBNext Ultra RNA Library Prep Kit for Illumina $185 $266 $333 $185 $266 $333 $186 $268 $334
RiboZero + NEBNext Ultra RNA Library Prep Kit for Illumina $278 $400 $501 $278 $400 $501 $279 $402 $502
NEBNext Multiplex Small RNA Library Prep Set for Illumina $195 $281 $352 $195 $281 $352 N/A N/A N/A
TruSeq Small RNA Sample Prep Kit $287 $413 $516 N/A N/A N/A N/A N/A N/A
Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis $314 $452 $565 N/A N/A N/A N/A N/A N/A
Fragmentation by Fragmentase $43 $62 $77 N/A N/A N/A N/A N/A N/A
Fragmentation by Covaris $44 $63 $80 N/A N/A N/A N/A N/A N/A