PRICING
Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for additional pricing information or Bioinformatics consulting.
Singe-cell library construction
| Internal (MGB) | External Academic | Industry | |
|---|---|---|---|
| 10X single-cell RNA-Seq library prep | $2348 | $3381 | $4226 |
| 10X single-cell ATAC-Seq library prep | $2529 | $3642 | $4553 |
| 10X single-cell CITE-Seq library prep | $2346 | $3378 | $4223 |
| 10X Single Cell Multiome ATAC + Gene Expression library prep | $3945 | $5681 | $7102 |
| 10X chip surcharge | $514 | $740 | $925 |
| 10X extraction of nuclei | $348 | $501 | $627 |
| 10X single-cell RNA-Seq 1-plex FLEX library prep from formaldehyde-fixed cells | $2473 | $3562 | $4452 |
| 10X single-cell RNA-Seq 4-plex FLEX library prep from formaldehyde-fixed cells | $4784 | $6889 | $8611 |
| 10X single-cell RNA-Seq 1-plex FLEX library prep from FFPE samples (cells resuspended by customer) | $2473 | $3562 | $4452 |
| 10X single-cell RNA-Seq 4-plex FLEX library prep from FFPE samples (cells resuspended by customer) | $4784 | $6889 | $8611 |
| 10X single-cell RNA-Seq 1-plex FLEX library prep from live or frozen cells | $2602 | $3746 | $4683 |
| 10X single-cell RNA-Seq 4-plex FLEX library prep from live or frozen cells | $5027 | $7239 | $9048 |
Library construction from bulk input
| Option | Internal (MGB) | External Academic | Industry |
|---|---|---|---|
| NEBNext Ultra II DNA Library Prep | $168 | $242 | $303 |
| NEBNext Multiplex Small RNA Library Prep | $239 | $344 | $430 |
| Clontech SMARTer Ultra Low Input RNA library prep v4 | $386 | $556 | $695 |
| NEBNext Single Cell/Low Input RNA Library Prep | $268 | $385 | $482 |
| NEBNext PolyA + NEBNext Ultra II RNA Library Prep | $231 |
$333 | $416 |
| NEBNext rRNA Depletion + NEBNext Ultra II RNA Library Prep | $299 |
$431 | $539 |
| Thruplex low-input DNA library prep | $186 | $269 | $336 |
| Clontech Pico RNA library prep | $425 | $612 | $765 |
| NEBNext blood Globin & rRNA Depletion + NEBNext UltraII Library Prep (see Norgen blood RNA extraction below) | $302 | $435 | $544 |
Related Services
| Internal (MGB) | External Academic | Industry | |
|---|---|---|---|
| Fragmentation by Covaris | $90 | $130 | $162 |
| Norgen blood RNA extraction from Paxgene tubes | $24 | $34 | $43 |
| Trizol RNA extraction from cell culture | $27 | $39 | $48 |
| Tapestation of DNA or RNA | $30 | $43 | $53 |
| Library quality control (required before sequencing) | $65 | $94 | $117 |
Sequencing settings
Illumina NextSeq 2000 instrument uses four types of sequencing settings: P1, P2, P3, P4 and can generate reads of various lengths. Libraries can be sequenced in either single-read (SR) or paired-end (PE) mode.
| Output, millions of reads | Available read lengths | |
|---|---|---|
| P1 | 100 | 100, 300, 600 |
| P2 | 440 | 100, 200, 300, 600 |
| P3 | 1100 | 50, 100, 200, 300 |
| P4 | 1800 | 50, 100, 200, 300 |
Illumina NextSeq 2000 sequencing
| Internal (MGB) | External Academic | Industry | |||||
|---|---|---|---|---|---|---|---|
| Run settings | Full flowcell | Cost per million reads | Full flowcell | Cost per million reads | Full flowcell | Cost per million reads | |
| P1 | PE50 | $1334 | $13.34 | $1921 | $19.21 | $2402 | $24.02 |
| P1 | SR100 | $1312 | $13.12 | $1889 | $18.89 | $2362 | $23.62 |
| P1 | PE150 | $1902 | $19.02 | $2739 | $27.39 | $3424 | $34.24 |
| P1 | PE300 | $2804 | $28.04 | $4038 | $40.38 | $5047 | $50.47 |
| P2 | PE50 | $1774 | $4.03 | $2555 | $5.81 | $3194 | $7.26 |
| P2 | SR100 | $1759 | $4.00 | $2532 | $5.76 | $3166 | $7.19 |
| P2 | PE100 | $3352 | $7.62 | $4826 | $10.97 | $6033 | $13.71 |
| P2 | SR200 | $3336 | $7.58 | $4804 | $10.92 | $6005 | $13.65 |
| P2 | PE150 | $4522 | $10.28 | $6512 | $14.80 | $8140 | $18.50 |
| P2 | PE300 | $5317 | $12.08 | $7656 | $17.40 | $9570 | $21.75 |
| P3 | PE25 | $2878 | $2.62 | $4144 | $3.77 | $5180 | $4.71 |
| P3 | SR50 | $2860 | $2.60 | $4119 | $3.74 | $5149 | $4.68 |
| P3 | PE50 | $3683 | $3.35 | $5304 | $4.82 | $6630 | $6.03 |
| P3 | SR100 | $3666 | $3.33 | $5279 | $4.80 | $6598 | $6.00 |
| P3 | PE100 | $5121 | $4.66 | $7375 | $6.70 | $9218 | $8.38 |
| P3 | SR200 | $5104 | $4.64 | $7349 | $6.68 | $9187 | $8.35 |
| P3 | PE150 | $6100 | $5.55 | $8784 | $7.99 | $10980 | $9.98 |
| P4 | PE25 | $2593 | $1.85 | $3734 | $2.67 | $4667 | $3.33 |
| P4 | SR50 | $2562 | $1.83 | $3689 | $2.63 | $4611 | $3.29 |
| P4 | PE50 | $3746 | $2.68 | $5394 | $3.85 | $6743 | $4.82 |
| P4 | SR100 | $3715 | $2.65 | $5349 | $3.82 | $6687 | $4.78 |
| P4 | PE100 | $5288 | $3.78 | $7615 | $5.44 | $9519 | $6.80 |
| P4 | SR200 | $5257 | $3.76 | $7570 | $5.41 | $9463 | $6.76 |
| P4 | PE150 | $6745 | $4.82 | $9713 | $6.94 | $12142 | $8.67 |
