PRICING
Overview
Basic pricing for NextGen library construction and sequencing services is given below. Additional fees apply for downstream Bioinformatics analysis services or consultations. Please contact us for specific pricing information on Bioinformatics consulting.
Price per lane of sequencing includes quality control of one library (or total of multiple librares if pooled by the customer), cluster generation, sequencing, demultiplexing of standard Illumina barcode reads, and online delivery of sequence and quality data. Prices for quality control of libraries after the first are given below. Prices for various types of library construction are also given below, and include validation of input DNA or RNA, library construction, and library validation.
In addition to high-throughput Illumina HiSeq sequencing in High-Output mode (150-200 million reads per lane) and Rapid mode (100-120 million reads per lane), we provide a lower-throughput but fast and less expensive MiSeq sequencing (10-15 million reads per run). For a MiSeq run, the customer should order and bring own Illumina sequencing kit that fits length and type requirements of the specific experiment. The service prices include charges per hour of MiSeq run, sequencing by technician, and sample quality control if required by the customer.
Illumina HiSeq Sequencing in High-Output mode
| Internal (Partners HealthCare) | External Academic | Industry | |
|---|---|---|---|
| Single 50 | $1240 | $1786 | $2232 |
| Paired 50 | $2080 | $2995 | $3744 |
| Paired 75 | $2360 | $3398 | $4248 |
| Single 100 | $1680 | $2419 |
$3024 |
| Paired 100 | $2630 | $3787 | $4734 |
| Paired 25 | $1600 | $2304 | $2880 |
Illumina HiSeq sequencing in Rapid mode
Illumina HiSeq2500 can perfom runs in Rapid mode, which are much faster but produce somewhat fewer reads (100-120 million reads per lane, two lanes per run). If both lanes of a rapid run include the same pool of samples, then cluster generation can be perfomed directly by HiSeq (on-board cluster generation, OBCG option). Otherwise, prior to the run we perform a separate step of cluster generation in the two lanes using the cBot instrument, similar to High-Output mode (cBot Duo option).
| Internal (Partners HealthCare) | External Academic | Industry | |
|---|---|---|---|
| Single 50 cBot Duo | $1290 | $1858 | $2322 |
| Single 50 OBCG | $1060 | $1526 | $1908 |
| Paired 50 cBot Duo | $1870 | $2693 | $3366 |
| Paired 50 OBCG | $1690 | $2434 | $3042 |
| Single 100 cBot Duo | $1520 | $2189 | $2736 |
| Single 100 OBCG | $1280 | $1843 | $2304 |
| Paired 100 cBot Duo | $2400 | $3456 | $4320 |
| Paired 100 OBCG | $2160 | $3110 | $3888 |
| Single 150 cBot Duo | $1770 | $2549 | $3186 |
| Single 150 OBCG | $1530 | $2203 | $2754 |
| Paired 150 cBot Duo | $2990 | $4306 | $5382 |
| Paired 150 OBCG | $2850 | $4104 | $5130 |
| Paired 25 cBot Duo | $1970 | $2837 | $3546 |
| Paired 25 OBCG | $1660 | $2390 | $2988 |
Illumina MiSeq Sequencing
MiSeq sequencing service includes instrument operation by technician and the charge for MiSeq use per hour. Quality control prices for more than one sample (if required) apply.
| Internal (Partners HealthCare) | External Academic | Industry | |
|---|---|---|---|
| Instrument operation by technician | $132 | $190 | $239 |
| MiSeq usage (cost per hour) | $30 | $43 | $55 |
Library Quality Control after the first library
| Internal (Partners HealthCare) | External Academic | Industry | |
|---|---|---|---|
| Price per library | $56 | $81 | $102 |
Single-Cell RNA-seq Library Construction (10x Genomics Chromium)
| Internal (Partners HealthCare) | External Academic | Industry | |
|---|---|---|---|
| Price per sample | $1565 | $2254 | $2818 |
Library Construction
| Option | Total cost per sample, SINGLE | External Academic | Industry | Total cost per sample, MULTI PLEXED | External Academic | Industry | Total cost per sample, DUAL INDEX | External Academic | Industry |
|---|---|---|---|---|---|---|---|---|---|
| NEBNext Ultra DNA Library Prep Kit for Illumina | $167 | $241 | $300 | $167 | $241 | $300 | $167 | $241 | $300 |
| NEBNext DNA Library Prep Master Mix Set for Illumina | $181 | $261 | $326 | $181 | $261 | $326 | $181 | $261 | $326 |
| NEBNext PolyA + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina | $193 |
$278 | $348 | $193 |
$278 | $348 | $193 | $278 | $348 |
| PolyA selection + NEBNext Ultra Directional RNA Library Prep Kit for Illumina | $209 | $301 | $376 | $209 | $301 | $376 | $210 | $302 | $377 |
| NEBNext rRNA Depletion Kit + NEBNext Ultra II Directional RNA Library Prep Kit for Illumina | $247 | $356 | $445 | $247 |
$356 | $445 | $247 | $356 | $445 |
| RiboZero + NEBNext Ultra Directional RNA Library Prep Kit for Illumina | $308 | $444 | $554 | $308 | $444 | $554 | $308 | $444 | $555 |
| NEBNext Multiplex Small RNA Library Prep Set for Illumina | $214 | $308 | $385 | $214 | $308 | $385 | N/A | N/A | N/A |
| TruSeq Small RNA Sample Prep Kit | $327 | $471 | $588 | $327 | $471 | $588 | N/A | N/A | N/A |
| NEBNext Single Cell/Low Input RNA Library Prep Kit For Illumina | $202 | $291 | $363 | $202 |
$291 | $363 | N/A | N/A | N/A |
| Clontech SMARTer Ultra Low Input RNA Kit for Sequencing v4 cDNA Synthesis for Single-Cell Transcriptome Analysis | $327 | $471 | $589 | $327 | $471 | $589 | N/A | N/A | N/A |
| Fragmentation by Fragmentase | $49 | $71 | $88 | N/A | N/A | N/A | N/A | N/A | N/A |
| Fragmentation by Covaris | $50 | $72 | $90 | N/A | N/A | N/A | N/A | N/A | N/A |
